Bad (phospho Ser134) rabbit pAb from ELK Biotechnology Read more ›
Bad (phospho Ser134) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Bad in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008],
| Specificity | Phospho-Bad (S134) Polyclonal Antibody detects endogenous levels of Bad protein only when phosphorylated at S134. |
|---|---|
| Validation evidence captions | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using BAD (Phospho-Ser134) Antibody | Immunofluorescence analysis of HeLa cells, using BAD (Phospho-Ser134) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human brain, using BAD (Phospho-Ser134) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from mouse liver, using BAD (Phospho-Ser134) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES6877 |
| Target | Bad |
| Research areas | >>EGFR tyrosine kinase inhibitor resistance, >>Endocrine resistance, >>Platinum drug resistance, >>ErbB signaling pathway, >>Ras signaling pathway, >>cGMP-PKG signaling pathway, >>cAMP signaling pathway, >>Chemokine signaling pathway, >>Autophagy - animal, >>PI3K-Akt signaling pathway, >>Apoptosis, >>VEGF signaling pathway, >>Focal adhesion, >>Neurotrophin signaling pathway, >>Insulin signaling pathway, >>Thyroid hormone signaling pathway, >>Alzheimer disease, >>Amyotrophic lateral sclerosis, >>Prion disease, >>Pathways of neurodegeneration - multiple diseases, >>Toxoplasmosis, >>Tuberculosis, >>Hepatitis C, >>Hepatitis B, >>Measles, >>Human papillomavirus infection, >>Herpes simplex virus 1 infection, >>Human immunodeficiency virus 1 infection, >>Pathways in cancer, >>Viral carcinogenesis, >>Chemical carcinogenesis - receptor activation, >>Chemical carcinogenesis - reactive oxygen species, >>Colorectal cancer, >>Renal cell carcinoma, >>Pancreatic cancer, >>Endometrial cancer, >>Prostate cancer, >>Melanoma, >>Chronic myelo |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human BAD around the phosphorylation site of Ser134. AA range:100-149 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in a wide variety of tissues. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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