Custom research services · ABMIUM
Custom PCR & qPCR Assay Development
Custom PCR, qPCR and RT-qPCR assay design and optimisation. Define primers, probes, sample matrix, instrument compatibility and validation requirements.
Specification reviewed before feasibility, pricing and lead time are confirmed.
Built around the experiment
Your project. A defined technical scope.
ABMIUM coordinates custom PCR and qPCR assay development around a defined target, reference sequence and research application. Projects may involve primer and probe design, an assay-development package or a specified reagent deliverable. The review considers sample type, instrument, chemistry and specificity requirements together. A clear reference sequence and control strategy help prevent an assay being developed for the wrong transcript, variant or genomic region.
ABMIUM reviews your specification and coordinates suitable custom-service and manufacturing routes. The proposed provider or manufacturer, available documentation and agreed scope are stated before order.
Choose the stages you need
Service modules
Start with one module or combine related stages into a clearly scoped project.
Target definition and primer/probe design
Provide gene symbol, transcript, accession, locus or reference genome build. State which isoforms or variants should be detected or excluded. Specify primer/probe...
Technical scope
Provide gene symbol, transcript, accession, locus or reference genome build. State which isoforms or variants should be detected or excluded. Specify primer/probe requirements and any existing design or sequence constraints.
PCR and qPCR chemistry
Discuss PCR, RT-PCR, qPCR or RT-qPCR, with dye-based or probe-based detection as appropriate. Include amplicon preferences, sample preparation and instrument channels. Multiplex...
Technical scope
Discuss PCR, RT-PCR, qPCR or RT-qPCR, with dye-based or probe-based detection as appropriate. Include amplicon preferences, sample preparation and instrument channels. Multiplex requirements should be reviewed with the complete target list.
Instrument and sample compatibility
State the platform, reaction volume and intended sample type. Define whether extracted DNA, RNA or another prepared input is supplied. Compatibility and...
Technical scope
State the platform, reaction volume and intended sample type. Define whether extracted DNA, RNA or another prepared input is supplied. Compatibility and interference testing should be included where they affect the research use.
Controls and research validation
Agree the experiments required to assess specificity, amplification efficiency, reproducibility and working range. Identify positive, negative and no-template controls, and reverse-transcription controls...
Technical scope
Agree the experiments required to assess specificity, amplification efficiency, reproducibility and working range. Identify positive, negative and no-template controls, and reverse-transcription controls where applicable. The deliverable and method documentation should match the commissioned validation scope.
Connect the service to your research
Research applications
Gene-expression research
Request a defined transcript-measurement assay with the reference, normalisation approach and input requirements stated.
Genotyping and variant research
Discuss sequence-discrimination requirements and relevant positive and negative control material.
Research target detection
Scope target detection in a specified experimental matrix and clarify any homologous sequences that could affect specificity.
Agree the output before work begins
Project deliverables
Material, experiments and documentation are defined in the quotation. Select the deliverables that your study needs.
- Primer/probe sets, assay reagents or a development package as quoted.
- Target and design documentation within the agreed disclosure scope.
- Performance data for the validation experiments commissioned.
- Method, handling and instrument-compatibility information where included.
One coordinated route
How your custom project works
- 01
Share your requirement
Tell us the target, application, starting materials and output you need. Include performance and documentation requirements.
- 02
Review feasibility and scope
ABMIUM coordinates the technical review and identifies the proposed provider, methods, deliverables and project stages.
- 03
Confirm the quotation
Review the agreed specification, available QC and reports, pricing, indicative lead time and delivery terms before order.
Clear answers before you enquire
Frequently asked questions
Can I request primers and probes without a complete kit?
Yes. Specify the required deliverable so design, synthesis and experimental testing can be separated in the quotation.
Can you develop a multiplex qPCR assay?
Include all targets and the instrument channels. Multiplex feasibility depends on the complete panel and needs a defined compatibility and validation plan.
Do I need to supply a genome build?
Supply an accession or reference build whenever sequence interpretation depends on it. This is particularly useful for genomic loci, variants and isoform-specific designs.
Can PCR services be used for diagnostic development?
State the intended use at enquiry. The service here is research-focused; diagnostic development requires a separately assessed regulatory and validation specification.
Should our project use PCR, qPCR or RT-qPCR?
The target and output determine the route. Endpoint PCR can address presence or amplicon generation; qPCR adds a quantitative readout. RNA measurement requires reverse transcription. Provide the sequence reference, specimen, expected abundance and decision the assay must support.
What controls should a custom qPCR project include?
Define no-template controls, relevant positive and negative material, extraction or inhibition controls and, where appropriate, a no-reverse-transcription control. Agree specificity, efficiency, linear range and repeatability studies. Instrument compatibility and analysis settings belong in the transfer specification.
What affects the cost of custom pcr & qpcr assay development?
Pricing depends on target count, primer and probe design, sample preparation, instrument requirements and validation depth. ABMIUM provides a project-specific quotation after reviewing the requirement. Ask for a breakdown of stages, deliverables, optional studies and further-work decision points so you can compare the total scope rather than an entry price alone.
Talk to ABMIUM
Request a technical review & quote
Share the information below to help us scope custom pcr & qpcr assay development.
- Target sequence, accession, transcript or genome build.
- Technology, chemistry, platform and application.
- Sample type, input amounts and available control material.
- Deliverable, reaction quantity, specificity and validation criteria.
info@abmium.com
+44 (0)203 824 9119
UK-based ABMIUM supports research enquiries from the UK, Europe and the United States. Include your destination country so material transfer, delivery and documentation requirements can be assessed. Research Use Only; feasibility and lead time are confirmed against the final specification.