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ionSIGHTSee the Whole Picture

An AI-driven native mass spectrometry platform that delivers direct, high-fidelity insights into protein structure and drug interactions.

About ionSIGHT

ionSIGHT is an Oxford University spinout

Deep expertise in native mass spectrometry, machine learning, and specialist glycoprotein analysis.

The platform helps researchers move beyond proxy readouts by delivering direct insight into protein structure, interaction behaviour, and functional integrity.

Native mass spectrometry analysis
nMS
Native mass spec
AI
ML-driven analysis
Mass Spec QC

Mass Spec QC for Expressed Proteins to Complex Biologics

A structured quality ladder that moves from identity confirmation to covalent structure review and functional native MS evidence.

Core QC 1

Confirm protein identity

Bottom-up proteomic analysis confirms protein identity but sacrifices higher-order structural information.

Best fit when the main question is whether the expected protein is present.
Advanced QC 3

Preserve covalent structure

Top-down intact mass analysis preserves covalent structure enabling characterization of proteoforms, isoforms, and post-translational modifications (PTMs).

Best fit when accurate mass, purity, truncations, isoforms, and PTMs need review.
Premium QC 5

Preserve function

Native mass spectrometry preserves non-covalent interactions and higher-order structure, enabling assessment of conformational integrity, ligand binding, protein-protein interactions, and other functional attributes, in addition to covalent structure, isoforms, and PTMs.

Best fit when functional integrity, complex formation, and conformational state are critical quality attributes.

Core QC sample requirements

Nanogram to picogram quantities of pure protein in solution or gel bands excised from Coomassie-stained SDS PAGE gels.

Core QC outputs

Protein identities as Mascot protein database search results including hit ranking, MOWSE statistical score and peptide MSMS coverage.

Advanced QC sample requirements

A few micrograms of pure protein in solution at approximately 1 mg per ml.

Advanced QC outputs

Annotated intact protein mass spectra indicating protein identity by accurate mass, purity, truncations, isoforms and post-translational modifications (if present) including relative quantitation.

Premium QC sample requirements

Typically 50 micrograms of pure protein in solution in a total volume of 75 ul. Fresh or snap-frozen and supplied on dry ice.

Premium QC outputs

Annotated native protein mass spectra as advanced QC but including stoichiometry, ligand binding, ligand identity, binding affinity, stable conformers, percent activity, hydrodynamic radius and thermal stability.

Choosing the right Mass Spec QC for your protein

  • Protein QC should be appropriate for the protein’s intended use and the time, effort and cost this entails.
  • While both Core QC and Advanced QC provide essential molecular characterization, they may not distinguish between proteins that are structurally intact and functionally active versus covalently intact and functionally impaired. Native MS uniquely enables direct interrogation of higher-order assembly states and interaction profiles associated with biological function.
  • For this reason, we recommend Premium QC for any expressed protein where functional integrity, complex formation, and conformational state are critical quality attributes.
ABMIUM Partnership

Structural insight at the point of purchase decision

ionSIGHT and ABMIUM share a common principle: researchers deserve better data before committing resource. Through this partnership, ionSIGHT's native MS analysis informs the protein characterisation context behind ABMIUM product listings.

For biotech and pharma teams sourcing reagents and biologics via ABMIUM, ionSIGHT represents an additional layer of independent scientific rigour from one of the most credentialed structural biology teams in Europe.

ABMIUM ValidatedTM standards: this service adds an independent native mass spectrometry evidence layer to support validation confidence, reagent selection, and structured technical review.
ionSIGHT adheres to responsible research standards consistent with the ABMIUM sustainability framework.
ABMIUM Validation Partner

What the ionSIGHT partnership adds to ABMIUM

Native MS data provides a dimension of structural truth that imaging and biochemical assays alone cannot capture.

  • Protein structure and interaction data at the product listing level
  • Independent native MS validation conducted by Oxford-trained structural biologists
  • Direct binding confirmation not inferred from proxy assays
  • Stoichiometry and selectivity
Get in touch

Discuss a protein QC or validation requirement

Share the scientific context, sample type, and decision you need the data to support. The team will review the request and respond by email.

Scientist reviewing mass spectrometry data

Make the enquiry easier to action

Tell us what you can about your protein

  • Core QC for protein identity questions
  • Advanced QC for intact mass and proteoform review
  • Premium QC for function-critical expressed proteins

Speak to a scientist

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Sent securely to the ABMIUM team for ionSIGHT follow-up.

See the whole picture with ionSIGHT

Native mass spectrometry at scale. AI-driven analysis. Direct observation of protein-drug interactions from one of the most credentialed structural biology teams in Europe.