ABMIUM
ELISA Analyser
Sandwich
Standard Series
Enter known concentrations & duplicate OD readings
| ID | Conc | OD Rep 1 | OD Rep 2 | Avg OD | Corr. OD |
|---|
Standard Curve
x = Corrected OD · y = Concentration
No curve data
Enter at least 3 standards with OD values
96-Well Plate Editor
Click a well · Tab/Enter to advance · Arrow keys to navigate
Assigning sample names
Standard
Blank
Sample
Core Definitions
Corrected OD
Sandwich/Direct/Indirect: Avg OD − Blank Avg OD. Competitive: Raw Avg OD (no subtraction).
Sandwich/Direct/Indirect: Avg OD − Blank Avg OD. Competitive: Raw Avg OD (no subtraction).
Standard Curve
2nd order polynomial: Conc = a·OD² + b·OD + c. R² must exceed 0.990 for valid results.
2nd order polynomial: Conc = a·OD² + b·OD + c. R² must exceed 0.990 for valid results.
CV%
SD of replicate concentrations ÷ mean × 100. CV% <15% acceptable. CV% >20% indicates variability.
SD of replicate concentrations ÷ mean × 100. CV% <15% acceptable. CV% >20% indicates variability.
OOR (Out of Range)
Sample OD outside the standard curve range. Re-assay at a different dilution.
Sample OD outside the standard curve range. Re-assay at a different dilution.
ELISA Format Comparison
| Format | Signal vs Conc | Blank Correction |
|---|---|---|
| Sandwich | Increases ↑ | Subtract blank OD |
| Direct | Increases ↑ | Subtract blank OD |
| Indirect | Increases ↑ | Subtract blank OD |
| Competitive | Decreases ↓ | Raw OD (no subtraction) |
Settings
Assay Information
Assay / Project Name
Analyst Name
Run Date
Notes
Assay Configuration
ELISA Type
Concentration Units
Acceptable CV% Threshold
Chart Appearance
Fitted curve colour
Standard point colour
Point size — 5px
Show grid lines
Show equation on chart
Data Management