Manufactured by:Reed Biotech
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Human VEGF VeriQuant Immunoassay Kit

Human VEGF VeriQuant Immunoassay Kit from Reed Biotech

SKU:
ABM223638-1
Manufacturer SKU
VQ1061H
Product type
Proximity-based Immunoassay
TargetVEGF
Species / reactivityHuman
Sample typeSerum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates, Other biological fluids

Manufacturer provenance

Manufactured by:
Reed Biotech
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

This protein is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This protein is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome.

Test principle

(a) Two monoclonal antibodies against distinct epitopes are conjugated with oligo probes, then form a sandwich complex with the antigen. (b) Following antigen recognition by the paired antibodies, the conjugated oligonucleotides are spatially proximate, and generate an amplifiable dsDNA template under the enzymatic action. (c) qPCR amplification is then performed. CT values correlate inversely with protein concentration, and sample target concentrations are calculated via standard curve.
Key facts & specifications
Also known as
N-VEGF, VEGFA, Vascular permeability factor, VPF, L-VEGF, Vascular endothelial growth factor A, long form
Assay type
Proximity-based Immunoassay
Detection method
qPCR
Sample volume
1~5μL
Assay instrument
qPCR Instruments
Manual handling time
20 min
Wash-free workflow
Yes
Signal label / dye
FAM™
Sensitivity
0.0704pg/mL
Detection range
0.128-2000 pg/mL
Wells / tests
96T
Assay duration
2 H
Quantity per pack
96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Manufacturer-listed assay compatibility

Evidence status by application and model.

Species / target matrixProtein quantification
Human

Sample type validation

Sample compatibility evidence using the same validation key.

Sample typeSerumPlasmaTissue homogenatesCell lysatesCell culture supernatesOther biological fluids
Sample compatibility
Identifiers & database references
Manufacturer SKU
VQ1061H
Gene ID
7422
UniProt accession
P15692
Storage, stability & shipping
Storage conditions
-20°C
Shipped at
Ultra-low temperature phase-change materials
Long-term storage
-20°C
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Reed Biotech and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Reed Biotech
£360.00£216.00