Human PSMa1 (Proteasome Subunit Alpha Type 1) Fast Step ELISA Kit

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PSMa1. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for Human PSMa1 are added to the micro ELISA plate wells. Human PSMa1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human PSMa1. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human PSMa1. You can calculate the concentration of Human PSMa1 in the samples by comparing the OD of the samples to the standard curve.