NEST® Multi-layer Cell Culture Flasks, TC-treated, Vent Cap

Multi-layer Cell Culture Flasks, TC-treated, Vent Cap from NEST Scientific for research and laboratory use.

Available configurations

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3-Layer Cell Culture Flask, 525 cm², Vent Cap, Straight Neck, TC, Sterile 1/pk, 12/cs

NEST Cat. No.: 731302
Selling unit: Case of 12

3-Layer Cell Culture Flask, 525 cm², Vent Cap, TC, Straight Neck, Sterile (Cat. No. 731302)

Key Features

Product Details

Quality Details

Nest 3-Layer Flasks offer a cost-effective solution for scaling up cell cultures while reducing time and effort in the lab. While you can’t speed up cell growth, you can minimize the hours spent in the tissue culture suite. Each multi-layer flask provides the growth surface equivalent of three 175 cm² flasks in a single, compact vessel. This design not only saves valuable incubator space but also enables you to cultivate up to three times as many cells within the same footprint.

What is the main use of the 731302 flask?

The 731302 is designed for routine adherent cell culture expansion with improved gas exchange for optimal cell growth.

Why does this flask use a vent cap?

The vent cap allows controlled airflow for CO₂/O₂ exchange, which supports healthier growth conditions for most standard cell lines.

What applications is it commonly used for?

It is widely used in R&D, seed train expansion, protein expression studies, and early-stage viral vector research.

Is this flask suitable for stem cell culture?

Yes, it is commonly used for MSC and iPSC expansion during early and mid-passages under standard culture conditions.

What makes the 3-layer format useful?

It increases surface area compared to traditional flasks while maintaining manageable handling and media exchange.

731301 3 Layer TC Treated Plug Cap N/A N/A N/A 132867 62407-079 Controlled handling, short-term culture, and transport-sensitive workflows

731002 5 Layer TC Treated Vent Cap 229325 25-151 353144 N/A 89204-478 Large-scale adherent cell production and biomanufacturing workflows

5-Layer Cell Culture Flask, Straight Neck, Vent Cap, TC Treated, 1/pk, 8/cs

NEST Cat. No.: 731002
Selling unit: Case of 8

5-Layer Cell Culture Flask, 825 cm², Vent Cap, TC, Straight Neck, Sterile (Cat. No. 731002)

Key Features

Product Details

Quality Details

Nest 5-Layer Flasks offer a cost-effective solution for scaling up cell cultures while reducing time and effort in the lab. While you can’t speed up cell growth, you can minimize the hours spent in the tissue culture suite. Each multi-layer flask provides the growth surface equivalent of five 175 cm² flasks in a single, compact vessel. This design not only saves valuable incubator space but also enables you to cultivate up to three times as many cells within the same footprint.

What is the 731002 flask used for?

The 731002 is designed for large-scale adherent cell culture with maximum gas exchange efficiency for high-density growth.

Why is a vent cap important for this flask?

The vent cap allows continuous CO₂/O₂ exchange, which is critical for maintaining healthy growth conditions in large surface area cultures.

What industries use this flask?

It is commonly used in biomanufacturing, vaccine production workflows, cell therapy development, and viral vector production.

How does this differ from 3-layer flasks?

The 5-layer design provides significantly higher surface area, making it more suitable for scale-up and production-level workflows.

Is this flask suitable for bioreactor preparation?

Yes, it is frequently used as an intermediate scale-up step before transferring cells into bioreactors.

731002 5 Layer TC Treated Vent Cap 229325 25-151 353144 N/A 89204-478 Large-scale adherent cell production and biomanufacturing workflows