NEST single-use bioprocess components and closed-system assemblies for sterile fluid transfer, media handling, sampling and cell-culture process connections.
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BioFactory, 40 Chamber, 2 Solid Overcaps, Culture Area 25,295cm2, TC Treated, Sterile, 1/PK, 2/CS from NEST Scientific for research and laboratory use.
NEST Cat. No.: 771422
Selling unit: Case of 2
Nest BioFactory 40 Chamber; 2 Solid Overcaps; Total culture area: 25295cm2; TC; Sterile (Cat.No. 771422)
| Specification | Listed detail |
|---|---|
| Detail | Forty-layer |
| Detail | 25295 cm² growth area |
| Packaging | 1 per bag, 2 units per case |
| Detail | TC treated - plasma etched for adherent cells |
| Detail | 2 Solid Overcaps attached |
| Detail | Also includes, 4 vented overcaps |
| Material | USP grade VI virgin polystyrene |
| Sterility | Sterile |
| Specification | Listed detail |
|---|---|
| Detail | Individually packaged in double-layer sterile bags |
Flask layers are ultrasonically welded together without adhesives or additives. This creates a strong, gas-tight seal between layers without introducing leachables and extractables that could cause cell culture abnormalities Two port mouth options available. Wide mouth (26 mm) ports make it easy to pour media and reach all layers of the flask. Narrow mouth (12 mm) ports are designed to fit aseptic transfer caps for sterile transfer of cells and media into and out of the flasks
Optional threaded caps are offered with chemically resistant, heat-sealable flexible TPE tubing, enabling aseptic transfer of media and cells
| Specification | Listed detail |
|---|---|
| Detail | Dimensionally and geometrically identical in design to Corning CellSTACK® flasks |
| Detail | Compatible with Corning accessories, caps and equipment |
| Detail | 100% of flasks are tested for leakage via pressure testing before shipping |
| Detail | Clear lot number for batch traceability |
| Detail | Suitable for large-scale cultivation of adherent cells |
| Detail | Large growth area—cultivate a high volume of cells in a single operation while minimizing the risk of contamination |
| Detail | Compatible with automated equipment. Especially important for larger 40-layer flasks |
| Detail | Sterility Guarantee — Utilizing an imported Rhodotron® TT200 electron accelerator from Belgian IBA and an in-house irradiation facility |
| Detail | Sterile to SAL 1 X 10^-6 |
Extensively tested and validated for leachables, extractables, particle counts, nucleases, nucelotides, endotoxins, pyrogens, TSE/BSE etc., making Nest Biofactories suitable for end point pharmaceutical manufacturing. Full validation dossiers are available on request
| Specification | Listed detail |
|---|---|
| Detail | Manufactured in cGMP (21 CFR part 820/821) compliant factory |
| Detail | Produced in ISO 13485:2016 and ISO 9001 facility |
| Detail | Molded and packed in ISO 7 cleanroom |
| Detail | E-beam sterilized and lot tested to SAL 10^-6 |
| Detail | Lot tested free of pyrogens USP 85 |
| Detail | Lot tested free of cytotoxins/pyrogens In Vivo USP 88 and In Vitro USP 87 |
| Detail | Lot tested free DNase/RNase and DNA |
| Detail | Lot tested free BSE/TSE |
| Detail | Certified free of animal products |
| Detail | Full validation dossier available upon request |
Nest Biofactory™ Systems are compact, multi-layer, single-use cell culture platforms designed for affordable and reliable cell culture scale-up. Ideal for medium- to large-scale research and industrial production, they support the production of cell-based products such as vaccines, monoclonal antibodies, proteins and other biopharmaceuticals. Nest Biofactories are based on the Corning CellSTACK® line of multi-layer tissue culture flasks. They are identical in geometry and dimensions for similar performance. However, Nest flasks are free of leachables and extractables that could cause cell culture irregularities. Layers are ultrasonically welded together without adhesives or additives, greatly improving lot to lot reproducibility.
A BioFactory is a multi-layer cell culture vessel designed to provide a large growth surface area in a compact footprint. BioFactories are commonly used for scaling adherent cell cultures for applications such as vaccine production, cell therapy, exosome manufacturing, viral vector production, and biologics development.
NEST BioFactories offer culture areas comparable to Corning CellSTACK vessels while providing multiple cap configurations, including wide plug seal, narrow plug seal, mixed cap, and solid overcap options. The chamber counts and growth areas closely match equivalent CellSTACK models.
NEST BioFactories are available in multiple sizes: Chambers Growth Area 1 Chamber 647 cm² 2 Chamber 1,279 cm² 5 Chamber 3,175 cm² 10 Chamber 6,335 cm² 40 Chamber 25,295 cm²
BioFactories are designed for adherent cell cultures, including: Mesenchymal stem cells (MSCs) HEK293 cells Vero cells CHO cells Fibroblasts Primary cells Induced pluripotent stem cells (iPSCs) Other anchorage-dependent mammalian cells
This product is manufactured by NEST® and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.