Manufactured by:Boster Bio
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Mouse Neutrophil Elastase/ELA2/ELANE ELISA Kit PicoKine®

ELISA Kit supplied by Boster Bio

SKU:
ABM33053
Manufacturer SKU
EK1445
Product type
ELISA Kits
TargetNeutrophil elastase
ApplicationsELISA
Species / reactivityMouse
Sample typeCell culture supernatant, Cell lysates, serum and plasma (heparin, EDTA).
PurityNatural and recombinant mouse Elane

Manufacturer provenance

Manufactured by:
Boster Bio
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Mouse Neutrophil Elastase/ELA2/ELANE ELISA Kit PicoKine® is a research-use ELISA kit from Boster Bio for measuring Neutrophil elastase in Cell culture supernatant, Cell lysates, serum and plasma (heparin, EDTA). samples from Mouse. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Mouse Neutrophil Elastase/ELA2/ELANE ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Elane in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Neutrophil elastase is a serine protease of neutrophil and monocyte granules. Its key physiologic role is in innate host defense, but it can also participate in tissue remodeling and possesses secretagogue actions important to local inflammatory responses. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.

Product information

Key facts & specifications
Also known as
Bone marrow serine protease; ELA2; ELANE; Elastase 2; elastase 2; neutrophil; GE; HLE; HNE; Human leukocyte elastase; Medullasin; NE; Neutrophil elastase; PMN E; PMN elastase
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Immunogen
Expression system for standard: NS0; Immunogen sequence: S27-R260
Sensitivity
<10 pg/ml
Detection range
31.2 pg/ml - 2,000 pg/ml
Sequence fragment
Expression system for standard: NS0; Immunogen sequence: S27-R260
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Sample type validation

Sample compatibility evidence using the same validation key.

Sample typeSample CompatibilityAssay RangeSensitivitySpecificity
cell culture supernatants
cell lysates
serum and plasma (heparin
EDTA).
Identifiers & database references
Manufacturer SKU
EK1445
Gene symbol
Elane
Gene ID
50701
UniProt accession
Q3UP87
Storage, stability & shipping
Shelf life
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Storage conditions
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Long-term storage
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Compliance & documents
CE marked
No
Datasheet
Open datasheet
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Boster Bio and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

ELANEelastase, neutrophil expressed

View target guide

ELANE identifies elastase, neutrophil expressed in Homo sapiens (Human). This target record connects NCBI Gene 1991, UniProtKB P08246 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
1991
UniProtKB
P08246
Boster Bio
£442.00£375.70