Mouse Ly9/SLAMF3/CD229 ELISA Kit PicoKine®

Mouse Ly9/SLAMF3/CD229 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ly9 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

T-lymphocyte surface antigen Ly-9 is a protein that in humans is encoded by the LY9 gene. This gene is mapped to 1 H3; 1 79.54 cM. Mature human CD229 consists of a 407 amino acid (aa) extracellular domain (ECD) with two Ig?like V-set and two Ig?like truncated C2-set domains. It also shows a 22 aa transmembrane segment, and a 179 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs. The ECD of human CD229 shares 57%?59% aa sequence identity with mouse and rat CD229. Within the first two Ig?like domains that are common to all SLAM proteins, human CD229 shares 24%?39% aa sequence identity with human 2B4, BLAME, CD2F-10, CD84, CRACC, NTB-A, and SLAM. Alternate splicing generates two additional isoforms that lack the juxtamembrane Ig?like domain or short cytoplasmic region. CD229 is expressed on T and B cells, thymocytes, and more weakly on NK cells. Homophilic binding between CD229 molecules is mediated by the N-terminal Ig?like domain. Human and mouse CD229 exhibit cross-species binding. Antigen stimulation of lymphocytes induces CD229 clustering to sites of T cell - B cell contact. Two tyrosines in the cytoplasmic domain are required for association of CD229 with the adaptor proteins AP-2 (μ2 chain) and GRB-2 and both are required for CD229 internalization.

Product information

Supplier	Boster Bio
ABMIUM evidence status	ABMIUM Verified™ collaborator-tested data
Catalogue reference	EK2011
Product category	PicoKine® ELISA Kits
Reactive species	Mouse
Tested applications	ELISA
Gene name	Ly9
Protein name	T-lymphocyte surface antigen Ly-9
Sample type	cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Assay range	93.7 pg/ml - 6,000 pg/ml
Sensitivity	<50 pg/ml
Specificity	Natural and recombinant mouse Ly9
Immunogen	Expression system for standard: NS0; Immunogen sequence: K48-R453
Storage	Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
UniProt	Q01965
NCBI Gene ID	17085

Available variants

Conjugation	Size
Not applicable	96 wells/kit, with removable strips.