Save 15%RUO

BRAF (7q34) Break-Apart FISH Probe Reagent

Research-use BRAF (7q34) break-apart FISH probe for studying genomic rearrangements involving BRAF (7q34) by fluorescence in situ hybridisation.

SKU:
ABM46296
Manufacturer SKU
FP-016
Product type
FISH Probe Reagent
TargetBRAF/7Q34
ApplicationsFISH
Sample typeTissue

Manufacturer provenance

Manufactured by:
Wuhan HealthCare Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Research-use BRAF (7q34) break-apart FISH probe for studying genomic rearrangements involving BRAF (7q34) by fluorescence in situ hybridisation.

The manufacturer describes a dual-colour break-apart design in which the BRAF 3′ region is labelled green and spans 200 kb, while the BRAF 5′ region is labelled orange and also spans 200 kb. The manufacturer probe map shows the two labelled regions flanking the BRAF locus with a short intervening segment.

Probe design

  • Target: BRAF
  • Locus: 7q34
  • Probe format: Break-apart
  • BRAF 3′ region: green, 200 kb
  • BRAF 5′ region: orange, 200 kb
  • Pack size: 100 µL/kit, 10 tests
  • Regulatory status: Research Use Only (RUO)
Wuhan HealthCare Biotechnology BRAF 7q34 break-apart probe design map
Manufacturer probe-design map for. Source: Wuhan HealthCare Biotechnology.

Research context

Wuhan HealthCare Biotechnology lists this probe under nervous-system and head-and-neck tumour applications and describes it for research into BRAF rearrangements such as translocations or inversions. ABMIUM presents those associations as research context only; the reagent is not supplied for clinical diagnosis.

Key facts & specifications
Research areas
Cytogenetics, Oncology research, Haematology research, Solid Tumors research
Also known as
BRAF (7q34) gene break apart probe reagent, BRAF (7q34)
Available conjugations
BRAF 5' orange, BRAF 3' green, orange, green, aqua, DAPI
Incubation temperature
42 °C
Assay type
Fluorescence in situ hybridisation (FISH)
Detection method
Fluorescence microscopy
Wells / tests
10 tests
Form / buffer
Ready-to-use FISH probe reagent
Sequence fragment
BRAF (7q34)
Quantity per pack
100µL/kit / 10 tests
Technical note
Probe design source text: BRAF gene 3' end labeled as green, with a length of 200 kb; BRAF gene 5' end labeled as orange, with a length of 200 kb. Protocol / imaging data: Hybridisation temperature: 42 °C; Hybridisation time: 2-16h; Denaturation temperature: 85°C; Denaturation time: 5min; Recommended imaging filters: DAPI + orange +green; Counterstain: DAPI; Signal interpretation: Refer to IFU; Expected normal signal pattern: Refer to IFU Label assignment: BRAF 5' orange, BRAF 3' green Cytoband(s): 7q34
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Sample type validation

Sample compatibility evidence using the same validation key.

Sample typeCompatible
Tissue
Identifiers & database references
Manufacturer SKU
FP-016
Storage, stability & shipping
Shelf life
12 months
Storage conditions
-20 °C ± 5 °C
Shipped at
below 0 °C
Short-term storage
below 0 °C
Long-term storage
-20 °C ± 5 °C
Storage warning
Protect fluorescent probe reagent from light during handling.
Compliance & documents
CE marked
No
Frequently asked questions
Who manufactured this product?

This product is manufactured by Wuhan HealthCare Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Wuhan HealthCare Biotechnology
£675.00£574.00