Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM100234

RL7A rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

RL7A rabbit pAb from ELK Biotechnology Read more ›

RL7A rabbit pAb
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RL7A rabbit pAb

Product Description

RL7A rabbit pAb is a research-use antibody from ELK Biotechnology for studying RL7A in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L7AE family of ribosomal proteins. It can interact with a subclass of nuclear hormone receptors, including thyroid hormone receptor, and inhibit their ability to transactivate by preventing their binding to their DNA response elements. This gene is included in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity. It is co-transcribed with the U24, U36a, U36b, and U36c small nucleolar RNA genes, which are located in its second, fifth, fourth, and sixth introns, respectively. This gene rearranges with the trk proto-oncogene t

Additional antibody information

Specificity RL7A Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: RL7A
Collaborator ELK Biotechnology
Partner model ES9329
Target RL7A
Research areas>>Ribosome, >>Coronavirus disease - COVID-19
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000 ELISA 1:5000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human protein . at AA range: 180-260
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system B-cell,Cerebellum,Eye,Lung,Placenta,Pooled,Skin,Uterus,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 ELISA 1:5000-20000
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
RL7A
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from human protein . at AA range: 180-260
Observed band
29kD
Cellular localization
nucleus,nucleolus,cytoplasm,cytosol,ribosome,cell-cell adherens junction,focal adhesion,membrane,cytosolic large ribosomal subunit,polysomal ribosome,extracellular exosome,
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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