Manufactured by:ELK Biotechnology
RUO

MYL2 rabbit pAb

MYL2 rabbit pAb from ELK Biotechnology

SKU:
ABM109803
Product type
Primary Antibody
TargetMYL2
ApplicationsWB, IHC, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemHighly expressed in type I muscle fibers.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

MYL2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying MYL2 in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity MYL2 Polyclonal Antibody detects endogenous levels of MYL2
Validation evidence captions Western Blot analysis of 1,mouse-heart 2,Hela cells using primary antibody diluted at 1:500(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour) | Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
Key facts & specifications
Research areas
>>Cardiac muscle contraction, >>Adrenergic signaling in cardiomyocytes, >>Apelin signaling pathway, >>Focal adhesion, >>Tight junction, >>Leukocyte transendothelial migration, >>Regulation of actin cytoskeleton, >>Shigellosis, >>Salmonella infection, >>Hypertrophic cardiomyopathy, >>Dilated cardiomyopathy
Also known as
Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC-2) (MLC-2v)
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen
Synthesized peptide derived from human MYL2. at AA range: 91-140
Observed band
18kD
Cellular localization
Cytoplasm, myofibril, sarcomere, A band .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 91-140
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES8862
UniProt entry
P10916
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

MYL2myosin light chain 2

View target guide

MYL2 identifies myosin light chain 2 in Homo sapiens (Human). This target record connects NCBI Gene 4633, UniProtKB P10916 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
4633
UniProtKB
P10916
ELK Biotechnology
£220.00