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Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99927

TNFAIP8L3 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

TNFAIP8L3 rabbit pAb from ELK Biotechnology Read more ›

TNFAIP8L3 rabbit pAb
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TNFAIP8L3 rabbit pAb

Product Description

TNFAIP8L3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying TNFAIP8L3 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

caution:It is uncertain whether Met-1 or Met-89 is the initiator.,similarity:Belongs to the TNFAIP8 family.,

Additional antibody information

Specificity This antibody detects endogenous levels of TNFAIP8L3
Validation evidence captions Western Blot analysis of 1,293T 2,mouse-brain cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)

Key Facts

Product typePrimary Antibody› Target: TNFAIP8L3
Collaborator ELK Biotechnology
Partner model ES8845
Target TNFAIP8L3
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000, ELISA 1:10000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human TNFAIP8L3. at AA range: 15-45
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Widely expressed (at protein level) (PubMed:25479791). Highly expressed in most carcinoma cell lines (PubMed:25479791, PubMed:25242044).
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 —
Rat — —
Mouse — —
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
TNFAIP8L3
Also known as
Tumor necrosis factor alpha-induced protein 8-like protein 3 (TNF alpha-induced protein 8-like protein 3) (TNFAIP8-like protein 3)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, ELISA 1:10000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from human TNFAIP8L3. at AA range: 15-45
Observed band
33kD
Cellular localization
Cytoplasm . Cell membrane . On PDGF activation, translocates from cytoplasm to plasma membrane. .
Dilution range
WB 1:500-2000, ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified™

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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