Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99925

LWS rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

LWS rabbit pAb from ELK Biotechnology Read more ›

LWS rabbit pAb
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LWS rabbit pAb

Product Description

LWS rabbit pAb is a research-use antibody from ELK Biotechnology for studying LWS in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

retinoid X receptor gamma(RXRG) Homo sapiens This gene encodes a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the antiproliferative effects of retinoic acid (RA). This receptor forms dimers with the retinoic acid, thyroid hormone, and vitamin D receptors, increasing both DNA binding and transcriptional function on their respective response elements. This gene is expressed at significantly lower levels in non-small cell lung cancer cells. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jun 2010],

Additional antibody information

Specificity This antibody detects endogenous levels of LWS
Validation evidence captions Western Blot analysis of 1,mouse-heart cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour) cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

Key Facts

Product typePrimary Antibody Target: LWS
Collaborator ELK Biotechnology
Partner model ES8842
Target LWS
Research areas>>PPAR signaling pathway, >>Th17 cell differentiation, >>Thyroid hormone signaling pathway, >>Adipocytokine signaling pathway, >>Parathyroid hormone synthesis, secretion and action, >>Pathways in cancer, >>Transcriptional misregulation in cancer, >>Chemical carcinogenesis - receptor activation, >>Thyroid cancer, >>Small cell lung cancer, >>Non-small cell lung cancer, >>Gastric cancer, >>Lipid and atherosclerosis
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000, ELISA 1:10000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human LWS. at AA range: 380-420
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in aortic endothelial cells (at protein level).
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
LWS
Also known as
Retinoic acid receptor RXR-gamma (Nuclear receptor subfamily 2 group B member 3) (Retinoid X receptor gamma)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, ELISA 1:10000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from human LWS. at AA range: 380-420
Observed band
50kD
Cellular localization
Nucleus . Cytoplasm .
Dilution range
WB 1:500-2000, ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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