Gremlin-2 rabbit pAb
Gremlin-2 rabbit pAb from ELK Biotechnology Read more ›
Product Description
Gremlin-2 rabbit pAb is an ELK Biotechnology antibody for research workflows involving Gremlin-2.
Background
This gene encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | The antibody detects endogenous Gremlin-2 |
|---|---|
| Validation evidence captions | Western blot analysis of RAT-brain lysate, antibody was diluted at 1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-small-intestine, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-small-intestine, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-colon-cancer, antibody was diluted at 1:200 |
Key Facts
| Target | Gremlin-2 |
| Also known as | Gremlin-2 (Cysteine knot superfamily 1, BMP antagonist 2;DAN domain family member 3;Protein related to DAN and cerberus) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000 |
| Species reactivity | Human;Mouse |
| Observed band | 19kD |
| Cellular localization | Secreted . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>TGF-beta signaling pathway |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000 |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human GREM2. AA range:71-120 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.