Gremlin-2 rabbit pAb from ELK Biotechnology Read more ›
Gremlin-2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Gremlin-2 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. [provided by RefSeq, Jul 2008],
| Specificity | The antibody detects endogenous Gremlin-2 |
|---|---|
| Validation evidence captions | Western blot analysis of RAT-brain lysate, antibody was diluted at 1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-small-intestine, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-small-intestine, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-colon-cancer, antibody was diluted at 1:200 |
| Collaborator | ELK Biotechnology |
| Partner model | ES8709 |
| Target | Gremlin-2 |
| Research areas | >>TGF-beta signaling pathway |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human GREM2. AA range:71-120 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Colon, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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