TGFβ2 rabbit pAb from ELK Biotechnology Read more ›
TGFβ2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying TGFβ2 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. Disruption of the TGF-beta/SMAD pathway has been implicated in a variety of human cancers. A chromosomal translocation that includes this gene is associated with Peters' anomaly, a congenital defect of the an
| Specificity | The antibody detects endogenous TGFβ2 |
|---|---|
| Validation evidence captions | Western blot analysis of mouse-kidney 293T mouse-lung lysate, antibody was diluted at 1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-placenta, antibody was diluted at 1:200 |
| Collaborator | ELK Biotechnology |
| Partner model | ES8706 |
| Target | TGFβ2 |
| Research areas | >>MAPK signaling pathway, >>Cytokine-cytokine receptor interaction, >>FoxO signaling pathway, >>Cell cycle, >>Cellular senescence, >>TGF-beta signaling pathway, >>Osteoclast differentiation, >>Hippo signaling pathway, >>AGE-RAGE signaling pathway in diabetic complications, >>Leishmaniasis, >>Chagas disease, >>Malaria, >>Toxoplasmosis, >>Amoebiasis, >>Tuberculosis, >>Hepatitis B, >>Human T-cell leukemia virus 1 infection, >>Pathways in cancer, >>Proteoglycans in cancer, >>MicroRNAs in cancer, >>Colorectal cancer, >>Renal cell carcinoma, >>Pancreatic cancer, >>Chronic myeloid leukemia, >>Hepatocellular carcinoma, >>Gastric cancer, >>Inflammatory bowel disease, >>Rheumatoid arthritis, >>Hypertrophic cardiomyopathy, >>Dilated cardiomyopathy, >>Diabetic cardiomyopathy |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TGF beta2. AA range:361-410 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Aorta endothelial cell,Lung, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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