Cystatin A rabbit pAb
Cystatin A rabbit pAb from ELK Biotechnology Read more ›
Product Description
Cystatin A rabbit pAb is an ELK Biotechnology antibody for research workflows involving Cystatin A.
Background
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | The antibody detects endogenous Cystatin A |
|---|---|
| Validation evidence captions | Western blot analysis of 293T mouse-kidney lysate, antibody was diluted at 1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-thymus, antibody was diluted at 1:200 |
Key Facts
| Target | Cystatin A |
| Also known as | Cystatin-A (Cystatin-AS;Stefin-A) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000 |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 11kD |
| Cellular localization | Cytoplasm . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000 |
| Immunogen | Synthetic peptide from human protein at AA range: 60-98 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.