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Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99868

DCT rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

DCT rabbit pAb from ELK Biotechnology Read more ›

DCT rabbit pAb
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DCT rabbit pAb

Product Description

DCT rabbit pAb is a research-use antibody from ELK Biotechnology for studying DCT in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

catalytic activity:L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.,cofactor:Binds 2 zinc ions per subunit.,function:Involved in regulating eumelanin and phaeomelanin levels.,pathway:Pigment biosynthesis; melanin biosynthesis.,similarity:Belongs to the tyrosinase family.,subunit:Tyrosinase, TYRP1 and TYRP2 may form a multienzyme complex.,

Additional antibody information

Specificity The antibody detects endogenous DCT
Validation evidence captions Western blot analysis of HELA A549 Cell Lysate, antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Key Facts

Product typePrimary Antibody› Target: DCT
Collaborator ELK Biotechnology
Partner model ES8594
Target DCT
Research areas>>Tyrosine metabolism, >>Metabolic pathways, >>Melanogenesis
Reactivity Human,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000, ELISA 1:10000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthetic peptide from human protein at AA range: 171-244
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Liver,Lymphnode melanoma metastases,Mammary gland,Melanoma,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 —
Mouse — —
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
DCT
Also known as
L-dopachrome tautomerase (DCT) (DT) (EC 5.3.3.12) (L-dopachrome Delta-isomerase) (Tyrosinase-related protein 2) (TRP-2) (TRP2)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, ELISA 1:10000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBELISA
Immunogen
Synthetic peptide from human protein at AA range: 171-244
Observed band
59kD
Cellular localization
Melanosome membrane ; Single-pass type I membrane protein . Melanosome . Proper trafficking to melanosome is regulated by SGSM2, ANKRD27, RAB9A, RAB32 and RAB38. .
Dilution range
WB 1:500-2000, ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified™

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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