ABM99867
IL-31 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
IL-31 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
IL-31 rabbit pAb is an ELK Biotechnology antibody for research workflows involving IL-31.
Background
IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA (MIM 609510) and OSMR (MIM 601743) that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma (Dillon et al., 2004 [PubMed 15184896]).[supplied by OMIM, Mar 2008],
Additional antibody information
| Specificity | The antibody detects endogenous IL-31 |
|---|---|
| Validation evidence captions | Western blot analysis of Hela Cell Lysate using antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-testis, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-testis, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200 |
Key Facts
Product type
Primary Antibody›
Target: IL-31
| Target | IL-31 |
| Also known as | Interleukin-31 (IL-31) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000, IHC-p 1:50-200 ELISA 1:10000-20000 |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 65kD |
| Cellular localization | Secreted . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Cytokine-cytokine receptor interaction |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | WB 1:500-2000, IHC-p 1:50-200 ELISA 1:10000-20000 |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human IL31. AA range:21-70 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
IL-31
Also known as
Interleukin-31 (IL-31)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, IHC-p 1:50-200 ELISA 1:10000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human IL31. AA range:21-70
Observed band
65kD
Cellular localization
Secreted .
Dilution range
WB 1:500-2000, IHC-p 1:50-200 ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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