TRPV4 rabbit pAb
TRPV4 rabbit pAb from ELK Biotechnology Read more ›
Product Description
TRPV4 rabbit pAb is an ELK Biotechnology antibody for research workflows involving TRPV4.
Background
transient receptor potential cation channel subfamily V member 4(TRPV4) Homo sapiens This gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Mutations in this gene are the cause of spondylometaphyseal and metatropic dysplasia and hereditary motor and sensory neuropathy type IIC. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010],
Additional antibody information
| Specificity | The antibody detects endogenous TRPV4 protein |
|---|---|
| Validation evidence captions | Western Blot analysis of HEPG2 cells using Antibody diluted at 500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
Key Facts
| Target | TRPV4 |
| Also known as | TRPV4 VRL2 VROAC |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000, ELISA 1:10000-20000 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 100kD |
| Cellular localization | Cell membrane . Apical cell membrane ; Multi-pass membrane protein . Cell junction, adherens junction . Cell projection, cilium . Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum. .; Isoform 1: Cell membrane .; Isoform 5: Cell membrane .; Isoform 2: Endoplasmic reticulum .; Isoform 4: Endoplasmic reticulum .; Isoform 6: Endoplasmic reticulum . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Cellular senescence;>>Inflammatory mediator regulation of TRP channels;>>Fluid shear stress and atherosclerosis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | WB 1:500-2000, ELISA 1:10000-20000 |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human TRPV4. AA range:461-510 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.