Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99859

TRPV4 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

TRPV4 rabbit pAb from ELK Biotechnology Read more ›

TRPV4 rabbit pAb 50µL/100µL
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TRPV4 rabbit pAb 50µL/100µL

Product Description

TRPV4 rabbit pAb is a research-use antibody from ELK Biotechnology for studying TRPV4 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

transient receptor potential cation channel subfamily V member 4(TRPV4) Homo sapiens This gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Mutations in this gene are the cause of spondylometaphyseal and metatropic dysplasia and hereditary motor and sensory neuropathy type IIC. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010],

Additional antibody information

Specificity The antibody detects endogenous TRPV4 protein
Validation evidence captions Western Blot analysis of HEPG2 cells using Antibody diluted at 500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Key Facts

Product typePrimary Antibody Target: TRPV4
Collaborator ELK Biotechnology
Partner model ES8551
Target TRPV4
Research areas>>Cellular senescence, >>Inflammatory mediator regulation of TRP channels, >>Fluid shear stress and atherosclerosis
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000, ELISA 1:10000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from the Internal region of human TRPV4. AA range:461-510
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Found in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level).
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
TRPV4
Also known as
TRPV4 VRL2 VROAC
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, ELISA 1:10000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human TRPV4. AA range:461-510
Observed band
100kD
Cellular localization
Cell membrane . Apical cell membrane ; Multi-pass membrane protein . Cell junction, adherens junction . Cell projection, cilium . Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum. .; Isoform 1: Cell membrane .; Isoform 5: Cell membrane .; Isoform 2: Endoplasmic reticulum .; Isoform 4: Endoplasmic reticulum .; Isoform 6: Endoplasmic reticulum .
Dilution range
WB 1:500-2000, ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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