Cytokeratin 14/17 rabbit pAb
Cytokeratin 14/17 rabbit pAb from ELK Biotechnology Read more ›
Product Description
Cytokeratin 14/17 rabbit pAb is an ELK Biotechnology antibody for research workflows involving Cytokeratin 14/17.
Background
This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | The antibody detects endogenous Cytokeratin 14/17 protein |
|---|---|
| Validation evidence captions | Western Blot analysis of HEPG2 cells using Antibody diluted at 1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:200 |
Key Facts
| Target | Cytokeratin 14/17 |
| Also known as | KRT14 |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000, ELISA 1:10000-20000 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 52kD |
| Cellular localization | Cytoplasm. Nucleus. Expressed in both as a filamentous pattern. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Estrogen signaling pathway;>>Staphylococcus aureus infection |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | WB 1:500-2000, ELISA 1:10000-20000 |
| Immunogen | Synthetic peptide from human protein at AA range: 261-310 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.