Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99843

ACSS1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

ACSS1 rabbit pAb from ELK Biotechnology Read more ›

ACSS1 rabbit pAb
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ACSS1 rabbit pAb

Product Description

ACSS1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ACSS1 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a mitochondrial acetyl-CoA synthetase enzyme. A similar protein in mice plays an important role in the tricarboxylic acid cycle by catalyzing the conversion of acetate to acetyl CoA. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011],

Additional antibody information

Specificity The antibody detects endogenous ACSS1 protein
Validation evidence captions Western Blot analysis of HEPG2 cells using Antibody diluted at 800. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Key Facts

Product typePrimary Antibody Target: ACSS1
Collaborator ELK Biotechnology
Partner model ES8528
Target ACSS1
Research areas>>Glycolysis / Gluconeogenesis, >>Pyruvate metabolism, >>Glyoxylate and dicarboxylate metabolism, >>Propanoate metabolism, >>Metabolic pathways, >>Carbon metabolism
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000, ELISA 1:10000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthetic peptide from human protein at AA range: 620-689
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Amygdala,Brain,PCR rescued clones,Placenta,Spleen,Stomach,T
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
ACSS1
Also known as
ACSS1 ACAS2L KIAA1846
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, ELISA 1:10000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
Synthetic peptide from human protein at AA range: 620-689
Observed band
75kD
Cellular localization
Mitochondrion matrix .
Dilution range
WB 1:500-2000, ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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