TRIF rabbit pAb from ELK Biotechnology Read more ›
TRIF rabbit pAb is a research-use antibody from ELK Biotechnology for studying TRIF in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes an adaptor protein containing a Toll/interleukin-1 receptor (TIR) homology domain, which is an intracellular signaling domain that mediates protein-protein interactions between the Toll-like receptors (TLRs) and signal-transduction components. This protein is involved in native immunity against invading pathogens. It specifically interacts with toll-like receptor 3, but not with other TLRs, and this association mediates dsRNA induction of interferon-beta through activation of nuclear factor kappa-B, during an antiviral immune response. [provided by RefSeq, Jan 2012],
| Specificity | TRIF Polyclonal Antibody detects endogenous levels of TRIF |
|---|---|
| Validation evidence captions | Western Blot analysis of HEPG2 cells using TRIF Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200 |
| Collaborator | ELK Biotechnology |
| Partner model | ES8501 |
| Target | TRIF |
| Research areas | >>NF-kappa B signaling pathway, >>Necroptosis, >>Toll-like receptor signaling pathway, >>NOD-like receptor signaling pathway, >>Alcoholic liver disease, >>Pertussis, >>Yersinia infection, >>Chagas disease, >>Hepatitis C, >>Hepatitis B, >>Influenza A, >>Human papillomavirus infection, >>Kaposi sarcoma-associated herpesvirus infection, >>Herpes simplex virus 1 infection, >>PD-L1 expression and PD-1 checkpoint pathway in cancer, >>Lipid and atherosclerosis |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000,IHC-p 1:50-200 ELISA 1:10000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from TRIF at AA range: 663-712 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Ubiquitously expressed but with higher levels in liver. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Rat | — | — |
| Mouse | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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