ABM99825
TRIF rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
TRIF rabbit pAb from ELK Biotechnology Read more ›
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Product Description
TRIF rabbit pAb is an ELK Biotechnology antibody for research workflows involving TRIF.
Background
This gene encodes an adaptor protein containing a Toll/interleukin-1 receptor (TIR) homology domain, which is an intracellular signaling domain that mediates protein-protein interactions between the Toll-like receptors (TLRs) and signal-transduction components. This protein is involved in native immunity against invading pathogens. It specifically interacts with toll-like receptor 3, but not with other TLRs, and this association mediates dsRNA induction of interferon-beta through activation of nuclear factor kappa-B, during an antiviral immune response. [provided by RefSeq, Jan 2012],
Additional antibody information
| Specificity | TRIF Polyclonal Antibody detects endogenous levels of TRIF |
|---|---|
| Validation evidence captions | Western Blot analysis of HEPG2 cells using TRIF Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200 |
Key Facts
Product type
Primary Antibody›
Target: TRIF
| Target | TRIF |
| Also known as | TIR domain-containing adapter molecule 1 (TICAM-1;Proline-rich, vinculin and TIR domain-containing protein B;Putative NF-kappa-B-activating protein 502H;Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta;TIR domain-containing adapter protein inducing IFN-beta) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000,IHC-p 1:50-200 ELISA 1:10000-20000 |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 80kD |
| Cellular localization | Cytoplasmic vesicle, autophagosome . Cytoplasm, cytosol . Mitochondrion . Colocalizes with UBQLN1 in the autophagosome (PubMed:21695056). Colocalizes in the cytosol with DDX1, DDX21 and DHX36. Colocalizes in the mitochondria with DDX1 and poly(I:C) RNA ligand. The multi-helicase-TICAM1 complex may translocate to the mitochondria upon poly(I:C) RNA ligand stimulation (By similarity). . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>NF-kappa B signaling pathway;>>Necroptosis;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>Alcoholic liver disease;>>Pertussis;>>Yersinia infection;>>Chagas disease;>>Hepatitis C;>>Hepatitis B;>>Influenza A;>>Human papillomavirus infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Herpes simplex virus 1 infection;>>PD-L1 expression and PD-1 checkpoint pathway in cancer;>>Lipid and atherosclerosis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | WB 1:500-2000,IHC-p 1:50-200 ELISA 1:10000-20000 |
| Immunogen | Synthesized peptide derived from TRIF at AA range: 663-712 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
TRIF
Also known as
TIR domain-containing adapter molecule 1 (TICAM-1;Proline-rich, vinculin and TIR domain-containing protein B;Putative NF-kappa-B-activating protein 502H;Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta;TIR domain-containing adapter protein inducing IFN-beta)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000,IHC-p 1:50-200 ELISA 1:10000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBIHCIFELISA
Immunogen
Synthesized peptide derived from TRIF at AA range: 663-712
Observed band
80kD
Cellular localization
Cytoplasmic vesicle, autophagosome . Cytoplasm, cytosol . Mitochondrion . Colocalizes with UBQLN1 in the autophagosome (PubMed:21695056). Colocalizes in the cytosol with DDX1, DDX21 and DHX36. Colocalizes in the mitochondria with DDX1 and poly(I:C) RNA ligand. The multi-helicase-TICAM1 complex may translocate to the mitochondria upon poly(I:C) RNA ligand stimulation (By similarity). .
Dilution range
WB 1:500-2000,IHC-p 1:50-200 ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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