Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99819

TRAF1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

TRAF1 rabbit pAb from ELK Biotechnology Read more ›

TRAF1 rabbit pAb
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TRAF1 rabbit pAb

Product Description

TRAF1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying TRAF1 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

TNF receptor associated factor 1(TRAF1) Homo sapiens The protein encoded by this gene is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from various receptors of the TNFR superfamily. This protein and TRAF2 form a heterodimeric complex, which is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF2 also interacts with inhibitor-of-apoptosis proteins (IAPs), and thus mediates the anti-apoptotic signals from TNF receptors. The expression of this protein can be induced by Epstein-Barr virus (EBV). EBV infection membrane protein 1 (LMP1) is found to interact with this and other TRAF proteins; this interaction is thought to link LMP1-mediated B lymphocyte transformation to the signal transduction from TNFR family receptors. Three transcript variants encoding two different isoforms have

Additional antibody information

Specificity TRAF1 Polyclonal Antibody detects endogenous levels of TRAF1
Validation evidence captions Western Blot analysis of HEPG2 cells using TRAF1 Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Key Facts

Product typePrimary Antibody Target: TRAF1
Collaborator ELK Biotechnology
Partner model ES8493
Target TRAF1
Research areas>>NF-kappa B signaling pathway, >>Apoptosis, >>TNF signaling pathway, >>Pathways in cancer, >>Transcriptional misregulation in cancer, >>Viral carcinogenesis, >>Small cell lung cancer
Reactivity Human,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000, ELISA 1:10000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from TRAF1 at AA range: 191-240
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Lymphoma,Skin,Spleen,Stomach,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
TRAF1
Also known as
TNF receptor-associated factor 1 (Epstein-Barr virus-induced protein 6)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, ELISA 1:10000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from TRAF1 at AA range: 191-240
Observed band
46kD
Cellular localization
cytoplasm,cytosol,
Dilution range
WB 1:500-2000, ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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