Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99812

Atg9a rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Atg9a rabbit pAb from ELK Biotechnology Read more ›

Atg9a rabbit pAb
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Atg9a rabbit pAb

Product Description

Atg9a rabbit pAb is a research-use antibody from ELK Biotechnology for studying Atg9a in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

function:Plays a role in autophagy.,similarity:Belongs to the ATG9 family.,

Additional antibody information

Specificity Atg9a Polyclonal Antibody detects endogenous levels of Atg9a
Validation evidence captions Western Blot analysis of 293T 3T3 cells using Atg9a Polyclonal Antibody diluted at 1:800. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Key Facts

Product typePrimary Antibody Target: Atg9a
Collaborator ELK Biotechnology
Partner model ES8476
Target Atg9a
Research areas>>Autophagy - other, >>Mitophagy - animal, >>Autophagy - animal
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000, ELISA 1:10000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthetic Peptide of Atg9a
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain,Embryo,Epithelium,Fetal brain,Lung endothelial cell,Lymph,Rectum tumor,Testis
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Atg9a
Also known as
ATG9 autophagy related 9 homolog A (S. cerevisiae)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, ELISA 1:10000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
Synthetic Peptide of Atg9a
Observed band
85kD
Cellular localization
Preautophagosomal structure membrane ; Multi-pass membrane protein . Cytoplasmic vesicle, autophagosome membrane ; Multi-pass membrane protein . Golgi apparatus, trans-Golgi network membrane ; Multi-pass membrane protein . Late endosome membrane ; Multi-pass membrane protein . Recycling endosome membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Mitochondrion membrane ; Multi-pass membrane protein . Mainly localizes to the trans-Golgi network (TGN) and the endosomal system; cycles between them though vesicle trafficking (PubMed:27316455, PubMed:27663665). Export from the TGN to promote formation of autophagosomes is mediated by the AP-4 complex (PubMed:29180427, PubMed:30262884). Under amino acid starvation or rapamycin treatment, redist
Dilution range
WB 1:500-2000, ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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