E-Selectin rabbit pAb
E-Selectin rabbit pAb from ELK Biotechnology Read more ›
Product Description
E-Selectin rabbit pAb is an ELK Biotechnology antibody for research workflows involving E-Selectin.
Background
The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | E-Selectin Polyclonal Antibody detects endogenous levels of E-Selectin |
|---|---|
| Validation evidence captions | Western Blot analysis of K562 SH-SY5Y mouse-brain cells using E-Selectin Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). |
Key Facts
| Target | E-Selectin |
| Also known as | selectin E |
| Concentration | 1 mg/ml |
| Working concentration | IHC-p: 100-300.WB 1:500-2000, ELISA 1:10000-20000 |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 66kD |
| Cellular localization | Cell membrane ; Single-pass type I membrane protein. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Cell adhesion molecules;>>TNF signaling pathway;>>AGE-RAGE signaling pathway in diabetic complications;>>African trypanosomiasis;>>Malaria;>>Lipid and atherosclerosis;>>Fluid shear stress and atherosclerosis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | IHC-p: 100-300.WB 1:500-2000, ELISA 1:10000-20000 |
| Immunogen | The antiserum was produced against synthesized peptide derived from the N-terminal region of human SELE. AA range:100-150 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | IHC-p: 100-300.WB 1:500-2000 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.