Vimentin (Phospho-Tyr38) rabbit pAb
Vimentin (Phospho-Tyr38) rabbit pAb from ELK Biotechnology Read more ›
Product Description
Vimentin (Phospho-Tyr38) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Vimentin.
Background
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009],
Additional antibody information
| Specificity | This antibody detects endogenous levels of Vimentin at Human:Y38;Mouse:Y38;Rat:Y38, It doesn't reacte with total protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of HELA cells using Antibody diluted at 500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Vimentin (Phospho-Ser38) Antibody | Western blot analysis of lysates from 293 cells treated with paclitaxel, using p-Vimentin (Phospho-Ser38) antibody. |
Key Facts
| Target | Vimentin |
| Also known as | VIM |
| Concentration | 1 mg/ml |
| Working concentration | WB: 1:500-10000 ELISA: 1:10000 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 51kD |
| Cellular localization | Cytoplasm . Cytoplasm, cytoskeleton . Nucleus matrix . Cell membrane . |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
| Research area | >>Epstein-Barr virus infection;>>MicroRNAs in cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | WB: 1:500-10000 ELISA: 1:10000 |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human Vimentin (Phospho-Tyr38) |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB: 1:500-10000 ELISA: 1:10000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.