Vimentin (Phospho-Tyr38) rabbit pAb from ELK Biotechnology Read more ›
Vimentin (Phospho-Tyr38) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Vimentin in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009],
| Specificity | This antibody detects endogenous levels of Vimentin at Human:Y38;Mouse:Y38;Rat:Y38, It doesn't reacte with total protein. |
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| Validation evidence captions | Western Blot analysis of HELA cells using Antibody diluted at 500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Vimentin (Phospho-Ser38) Antibody | Western blot analysis of lysates from 293 cells treated with paclitaxel, using p-Vimentin (Phospho-Ser38) antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES8459 |
| Target | Vimentin |
| Research areas | >>Epstein-Barr virus infection, >>MicroRNAs in cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB: 1:500-10000 ELISA: 1:10000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human Vimentin (Phospho-Tyr38) |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB: 1:500-10000 ELISA: 1:10000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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