E2F-1 (Acetyl Lys120) rabbit pAb from ELK Biotechnology Read more ›
E2F-1 (Acetyl Lys120) rabbit pAb is a research-use antibody from ELK Biotechnology for studying E2F-1 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can media
| Specificity | This antibody detects endogenous levels of E2F-1 at Human:K120;Mouse:K115;Rat:K118, It doesn't reacte with total protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of MOUSE-BRIAN cells using Antibody diluted at 2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
| Collaborator | ELK Biotechnology |
| Partner model | ES8446 |
| Target | E2F-1 |
| Research areas | >>Endocrine resistance, >>Cell cycle, >>Mitophagy - animal, >>Cellular senescence, >>Cushing syndrome, >>Hepatitis C, >>Hepatitis B, >>Human cytomegalovirus infection, >>Human papillomavirus infection, >>Human T-cell leukemia virus 1 infection, >>Kaposi sarcoma-associated herpesvirus infection, >>Epstein-Barr virus infection, >>Pathways in cancer, >>MicroRNAs in cancer, >>Chemical carcinogenesis - receptor activation, >>Pancreatic cancer, >>Glioma, >>Prostate cancer, >>Melanoma, >>Bladder cancer, >>Chronic myeloid leukemia, >>Small cell lung cancer, >>Non-small cell lung cancer, >>Breast cancer, >>Hepatocellular carcinoma, >>Gastric cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB: 1:500-10000 ELISA: 1:10000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized acetyl-peptide from human protein at AA range: 100-170 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Epithelium,Pancreas,Skin, |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB: 1:500-10000 ELISA: 1:10000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
No customer reviews yet.
