E2F-1 (Acetyl Lys120) rabbit pAb
E2F-1 (Acetyl Lys120) rabbit pAb from ELK Biotechnology Read more ›
Product Description
E2F-1 (Acetyl Lys120) rabbit pAb is an ELK Biotechnology antibody for research workflows involving E2F-1.
Background
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can media
Additional antibody information
| Specificity | This antibody detects endogenous levels of E2F-1 at Human:K120;Mouse:K115;Rat:K118, It doesn't reacte with total protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of MOUSE-BRIAN cells using Antibody diluted at 2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
Key Facts
| Target | E2F-1 |
| Also known as | E2F1 RBBP3 |
| Concentration | 1 mg/ml |
| Working concentration | WB: 1:500-10000 ELISA: 1:10000 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 60kD |
| Cellular localization | Nucleus . |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
| Research area | >>Endocrine resistance;>>Cell cycle;>>Mitophagy - animal;>>Cellular senescence;>>Cushing syndrome;>>Hepatitis C;>>Hepatitis B;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Human T-cell leukemia virus 1 infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Epstein-Barr virus infection;>>Pathways in cancer;>>MicroRNAs in cancer;>>Chemical carcinogenesis - receptor activation;>>Pancreatic cancer;>>Glioma;>>Prostate cancer;>>Melanoma;>>Bladder cancer;>>Chronic myeloid leukemia;>>Small cell lung cancer;>>Non-small cell lung cancer;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | WB: 1:500-10000 ELISA: 1:10000 |
| Immunogen | Synthesized acetyl-peptide from human protein at AA range: 100-170 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB: 1:500-10000 ELISA: 1:10000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.