p300 (Acetyl Lys1558/Acetyl Lys1560) rabbit pAb from ELK Biotechnology Read more ›
p300 (Acetyl Lys1558/Acetyl Lys1560) rabbit pAb is a research-use antibody from ELK Biotechnology for studying p300 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
E1A binding protein p300(EP300) Homo sapiens This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008],
| Specificity | Acetyl-p300 (K1558/K1560) Polyclonal Antibody detects endogenous levels of p300 around the acetylation site of K1558 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of HELA 293T using Acetyl-p300 (K1558/K1560) antibody. Antibody was diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
| Collaborator | ELK Biotechnology |
| Partner model | ES8433 |
| Target | p300 |
| Research areas | >>Viral life cycle - HIV-1, >>cAMP signaling pathway, >>HIF-1 signaling pathway, >>FoxO signaling pathway, >>Cell cycle, >>Wnt signaling pathway, >>Notch signaling pathway, >>TGF-beta signaling pathway, >>Adherens junction, >>JAK-STAT signaling pathway, >>Long-term potentiation, >>Melanogenesis, >>Thyroid hormone signaling pathway, >>Glucagon signaling pathway, >>Growth hormone synthesis, secretion and action, >>Huntington disease, >>Tuberculosis, >>Hepatitis B, >>Influenza A, >>Human papillomavirus infection, >>Human T-cell leukemia virus 1 infection, >>Kaposi sarcoma-associated herpesvirus infection, >>Pathways in cancer, >>Viral carcinogenesis, >>MicroRNAs in cancer, >>Renal cell carcinoma, >>Prostate cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized acetyl-peptide derived from human p300 around the acetylation site of K1558. |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Epithelium,Skin, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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