ABM99784
CD56 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
CD56 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
CD56 rabbit pAb is an ELK Biotechnology antibody for research workflows involving CD56.
Background
This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011],
Additional antibody information
| Specificity | CD56 Polyclonal Antibody detects endogenous levels of CD56 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of mouse-lung mouse-heart lysis using NCAM1 antibody. Antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200 | Western Blot analysis of Hela cells using Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
Key Facts
Product type
Primary Antibody›
Target: CD56
| Target | CD56 |
| Also known as | NCAM1; NCAM; Neural cell adhesion molecule 1; N-CAM-1; NCAM-1; CD56 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 94kD |
| Cellular localization | Isoform 1: Cell membrane; Single-pass type I membrane protein.; Isoform 2: Cell membrane; Single-pass type I membrane protein.; Isoform 3: Cell membrane; Lipid-anchor, GPI-anchor.; Isoform 4: Cell membrane ; Lipid-anchor, GPI-anchor .; Isoform 5: Secreted.; Isoform 6: Secreted . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Cell adhesion molecules;>>Prion disease |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human NCAM1. AA range:250-300 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
CD56
Also known as
NCAM1; NCAM; Neural cell adhesion molecule 1; N-CAM-1; NCAM-1; CD56
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human NCAM1. AA range:250-300
Observed band
94kD
Cellular localization
Isoform 1: Cell membrane; Single-pass type I membrane protein.; Isoform 2: Cell membrane; Single-pass type I membrane protein.; Isoform 3: Cell membrane; Lipid-anchor, GPI-anchor.; Isoform 4: Cell membrane ; Lipid-anchor, GPI-anchor .; Isoform 5: Secreted.; Isoform 6: Secreted .
Dilution range
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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