Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99770

CD88 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

CD88 rabbit pAb from ELK Biotechnology Read more ›

CD88 rabbit pAb
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CD88 rabbit pAb

Product Description

CD88 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CD88 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

function:Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.,PTM:Sulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.,similarity:Belongs to the G-protein coupled receptor 1 family.,

Additional antibody information

Specificity CD88 Polyclonal Antibody detects endogenous levels of CD88 protein.
Validation evidence captions Western blot analysis of MOUSE-BRAIN RAT-SPLEEN lysis using C5AR1 antibody. Antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western Blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Key Facts

Product typePrimary Antibody Target: CD88
Collaborator ELK Biotechnology
Partner model ES8389
Target CD88
Research areas>>Neuroactive ligand-receptor interaction, >>Complement and coagulation cascades, >>Neutrophil extracellular trap formation, >>Alcoholic liver disease, >>Staphylococcus aureus infection, >>Coronavirus disease - COVID-19
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from the N-terminal region of human C5AR1. AA range:1-50
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
CD88
Also known as
C5AR1; C5AR; C5R1; C5a anaphylatoxin chemotactic receptor; C5a-R; C5aR; CD88
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from the N-terminal region of human C5AR1. AA range:1-50
Observed band
39kD
Cellular localization
Cell membrane ; Multi-pass membrane protein . Cytoplasmic vesicle . Phosphorylated C5aR colocalizes with ARRB1 and ARRB2 in cytoplasmic vesicles. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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