Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99747

IP3R-I rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

IP3R-I rabbit pAb from ELK Biotechnology Read more ›

IP3R-I rabbit pAb 50µL/100µL
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IP3R-I rabbit pAb 50µL/100µL

Product Description

IP3R-I rabbit pAb is a research-use antibody from ELK Biotechnology for studying IP3 Receptor in WB, IHC workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes an intracellular receptor for inositol 1,4,5-trisphosphate. Upon stimulation by inositol 1,4,5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum. Mutations in this gene cause spinocerebellar ataxia type 15, a disease associated with an heterogeneous group of cerebellar disorders. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Nov 2009],

Additional antibody information

Specificity IP3R-I Polyclonal Antibody detects endogenous levels of IP3R-I protein.
Validation evidence captions Western blot analysis of 293T using IP3R-I antibody. Antibody was diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product typePrimary Antibody Target: IP3 Receptor
Collaborator ELK Biotechnology
Partner model ES8348
Target IP3 Receptor
Research areas>>Calcium signaling pathway, >>cGMP-PKG signaling pathway, >>Phosphatidylinositol signaling system, >>Oocyte meiosis, >>Autophagy - animal, >>Apoptosis, >>Cellular senescence, >>Vascular smooth muscle contraction, >>Apelin signaling pathway, >>Gap junction, >>Platelet activation, >>NOD-like receptor signaling pathway, >>C-type lectin receptor signaling pathway, >>Circadian entrainment, >>Long-term potentiation, >>Retrograde endocannabinoid signaling, >>Glutamatergic synapse, >>Cholinergic synapse, >>Serotonergic synapse, >>Dopaminergic synapse, >>Long-term depression, >>Inflammatory mediator regulation of TRP channels, >>GnRH signaling pathway, >>Estrogen signaling pathway, >>Thyroid hormone synthesis, >>Oxytocin signaling pathway, >>Glucagon signaling pathway, >>Renin secretion, >>Aldosterone synthesis and secretion, >>Cortisol synthesis and secretion, >>Parathyroid hormone synthesis, secretion and action, >>GnRH secretion, >>Cushing syndrome, >>Growth hormone synthesis, >>Salivary secretion, >>Ga
Reactivity Human,Mouse,Rat
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:500-2000;IHC-p 1:50-300
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from IP3R-I . at AA range: 1700-1780
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Widely expressed.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300
Mouse
Rat
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
IP3 Receptor
Also known as
ITPR1; INSP3R1; Inositol 1,4,5-trisphosphate receptor type 1; IP3 receptor isoform 1; IP3R 1; InsP3R1; Type 1 inositol 1,4,5-trisphosphate receptor; Type 1 InsP3 receptor
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBIHC
Immunogen
Synthesized peptide derived from IP3R-I . at AA range: 1700-1780
Observed band
320kD
Cellular localization
Endoplasmic reticulum membrane ; Multi-pass membrane protein . Cytoplasmic vesicle, secretory vesicle membrane ; Multi-pass membrane protein . Cytoplasm, perinuclear region . Endoplasmic reticulum and secretory granules (By similarity). .
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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