ACYP2 rabbit pAb
ACYP2 rabbit pAb from ELK Biotechnology Read more ›
Product Description
ACYP2 rabbit pAb is an ELK Biotechnology antibody for research workflows involving ACYP2.
Background
Acylphosphatase can hydrolyze the phosphoenzyme intermediate of different membrane pumps, particularly the Ca2+/Mg2+-ATPase from sarcoplasmic reticulum of skeletal muscle. Two isoenzymes have been isolated, called muscle acylphosphatase and erythrocyte acylphosphatase on the basis of their tissue localization. This gene encodes the muscle-type isoform (MT). An increase of the MT isoform is associated with muscle differentiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2016],
Additional antibody information
| Specificity | ACYP2 Polyclonal Antibody detects endogenous levels of ACYP2 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°over night |
Key Facts
| Target | ACYP2 |
| Also known as | ACYP2; ACYP; Acylphosphatase-2; Acylphosphatase; muscle type isozyme; Acylphosphate phosphohydrolase 2 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Rat |
| Observed band | 18kD |
| Cellular localization | mitochondrion, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Pyruvate metabolism;>>Metabolic pathways |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | Synthesized peptide derived from ACYP2 . at AA range: 40-120 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.