CD40 (phospho Thr254) rabbit pAb from ELK Biotechnology Read more ›
CD40 (phospho Thr254) rabbit pAb is a research-use antibody from ELK Biotechnology for studying CD40 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIG
| Specificity | Phospho-CD40 (T254) Polyclonal Antibody detects endogenous levels of CD40 protein only when phosphorylated at T254. |
|---|---|
| Validation evidence captions | Western Blot analysis of COLO cells using Phospho-CD40 (T254) Polyclonal Antibody diluted at 1:500 | Western blot analysis of lysates from COLO205 cells treated with Insulin 0.01U/ml 15', using TNFRSF5 (Phospho-Thr254) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES8067 |
| Target | CD40 |
| Research areas | >>Cytokine-cytokine receptor interaction, >>NF-kappa B signaling pathway, >>Cell adhesion molecules, >>Toll-like receptor signaling pathway, >>Intestinal immune network for IgA production, >>Malaria, >>Toxoplasmosis, >>Human T-cell leukemia virus 1 infection, >>Epstein-Barr virus infection, >>Transcriptional misregulation in cancer, >>Asthma, >>Autoimmune thyroid disease, >>Systemic lupus erythematosus, >>Allograft rejection, >>Primary immunodeficiency, >>Viral myocarditis, >>Lipid and atherosclerosis |
| Reactivity | Human,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TNFRSF5 around the phosphorylation site of Thr254. AA range:220-269 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | B-cells and in primary carcinomas. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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