Nopp140 rabbit pAb
Nopp140 rabbit pAb from ELK Biotechnology Read more ›
Product Description
Nopp140 rabbit pAb is an ELK Biotechnology antibody for research workflows involving Nopp140.
Background
function:Related to nucleologenesis, may play a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. May play an important role in transcription catalyzed by RNA polymerase I.,PTM:Undergoes rapid and massive phosphorylation/dephosphorylation cycles on CK2 and PKC sites. There is evidence suggesting that CDC2 kinase phosphorylates p130 at the M-phase.,similarity:Contains 1 LisH domain.,subcellular location:Shuttles between the nucleolus and the cytoplasm. At telophase it begins to assemble into granular-like pre-nucleolar bodies which are subsequently relocated to nucleoli at the early G1-phase.,subunit:Interacts with RNA polymerase I 194 kDa subunit (RPA194) and with casein kinase-II.,
Additional antibody information
| Specificity | Nopp140 Polyclonal Antibody detects endogenous levels of Nopp140 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of various lysis using Nopp140 Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
Key Facts
| Target | Nopp140 |
| Also known as | NOLC1; KIAA0035; NS5ATP13; Nucleolar and coiled-body phosphoprotein 1; 140 kDa nucleolar phosphoprotein; Nopp140; Hepatitis C virus NS5A-transactivated protein 13; HCV NS5A-transactivated protein 13; Nucleolar 130 kDa protein; Nucleolar pho |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 74,130KD(Nucleolar phosphoprotein p130) |
| Cellular localization | Nucleus, nucleolus . Cytoplasm . Shuttles between the nucleolus and the cytoplasm. At telophase it begins to assemble into granular-like pre-nucleolar bodies which are subsequently relocated to nucleoli at the early G1-phase. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen | Synthesized peptide derived from Nopp140 . at AA range: 620-700 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.