Per2 (phospho Ser662) rabbit pAb from ELK Biotechnology Read more ›
Per2 (phospho Ser662) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Per2 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene may increase the risk of getting certain cancers and have been linked to sleep disorders. [provided by RefSeq, Jan 2014],
| Specificity | Phospho-Per2 (S662) Polyclonal Antibody detects endogenous levels of Per2 protein only when phosphorylated at S662. |
|---|---|
| Validation evidence captions | Western Blot analysis of 3T3 cells using Phospho-Per2 (S662) Polyclonal Antibody diluted at 1:500 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Period Circadian Protein 2 (Phospho-Ser662) Antibody | Immunohistochemistry analysis of paraffin-embedded human brain, using Period Circadian Protein 2 (Phospho-Ser662) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from NIH/3T3 cells treated with PMA 125ng/ml 30', using Period Circadian Protein 2 (Phospho-Ser662) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES7896 |
| Target | Per2 |
| Research areas | >>Circadian rhythm, >>Circadian entrainment, >>Transcriptional misregulation in cancer, >>Acute myeloid leukemia |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Period Circadian Protein 2 around the phosphorylation site of Ser662. AA range:636-685 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Widely expressed. Found in heart, brain, placenta, lung, liver, skeleatal muscle, kidney and pancreas. High levels in skeletal muscle and pancreas. Low levels in lung. Isoform 2 is expressed in keratinocytes (at protein level). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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