PEA-15 rabbit pAb from ELK Biotechnology Read more ›
PEA-15 rabbit pAb is a research-use antibody from ELK Biotechnology for studying PEA-15 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
phosphoprotein enriched in astrocytes 15(PEA15) Homo sapiens This gene encodes a death effector domain-containing protein that functions as a negative regulator of apoptosis. The encoded protein is an endogenous substrate for protein kinase C. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014],
| Specificity | PEA-15 Polyclonal Antibody detects endogenous levels of PEA-15 protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of HeLa cells, using PEA-15 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PEA-15 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from Jurkat cells, treated with PMA 125ng/ml 30', using PEA-15 Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES7856 |
| Target | PEA-15 |
| Research areas | Antibody Research |
| Reactivity | Human,Mouse,Rat,Monkey |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PEA-15. AA range:81-130 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Ubiquitously expressed. Most abundant in tissues such as heart, brain, muscle and adipose tissue which utilize glucose as an energy source. Lower expression in glucose-producing tissues. Higher levels of expression are found in tissues from individuals with type 2 diabetes than in controls. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Monkey | — | — |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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