Cbl (phospho Tyr774) rabbit pAb
Cbl (phospho Tyr774) rabbit pAb from ELK Biotechnology Read more ›
Product Description
Cbl (phospho Tyr774) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Cbl.
Background
Cbl proto-oncogene(CBL) Homo sapiens This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia, and expansion of CGG repeats in the 5' UTR has been associated with Jacobsen syndrome. Mutations in this gene are also the cause of Noonan syndrome-like disorder. [provided by RefSeq, Jul 2016],
Additional antibody information
| Specificity | Phospho-Cbl (Y774) Polyclonal Antibody detects endogenous levels of Cbl protein only when phosphorylated at Y774. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of A549 cells, using CBL (Phospho-Tyr774) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CBL (Phospho-Tyr774) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from HeLa cells treated with EGF 200ng/ml 30', using CBL (Phospho-Tyr774) Antibody. The lane on the right is blocked with the phospho peptide. |
Key Facts
| Target | Cbl |
| Also known as | CBL; CBL2; RNF55; E3 ubiquitin-protein ligase CBL; Casitas B-lineage lymphoma proto-oncogene; Proto-oncogene c-Cbl; RING finger protein 55; Signal transduction protein CBL |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 100kD |
| Cellular localization | Cytoplasm. Cell membrane. Cell projection, cilium . Golgi apparatus . Colocalizes with FGFR2 in lipid rafts at the cell membrane. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>ErbB signaling pathway;>>Ubiquitin mediated proteolysis;>>Endocytosis;>>Insulin signaling pathway;>>Bacterial invasion of epithelial cells;>>Pathways in cancer;>>Proteoglycans in cancer;>>Chronic myeloid leukemia |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CBL around the phosphorylation site of Tyr774. AA range:740-789 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.