Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99611

CPZ rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

CPZ rabbit pAb from ELK Biotechnology Read more ›

CPZ rabbit pAb
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CPZ rabbit pAb

Product Description

CPZ rabbit pAb is a research-use antibody from ELK Biotechnology for studying CPZ in WB, ELISA workflows with listed reactivity for Human, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

carboxypeptidase Z(CPZ) Homo sapiens This gene encodes a member of the metallocarboxypeptidase family. This enzyme displays carboxypeptidase activity towards substrates with basic C-terminal residues. It is most active at neutral pH and is inhibited by active site-directed inhibitors of metallocarboxypeptidases. Alternative splicing in the coding region results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity CPZ Polyclonal Antibody detects endogenous levels of CPZ protein.
Validation evidence captions Western Blot analysis of HEK293 lysis, using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000

Key Facts

Product typePrimary Antibody Target: CPZ
Collaborator ELK Biotechnology
Partner model ES7810
Target CPZ
Research areasAntibody Research
Reactivity Human,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from CPZ . at AA range: 330-410
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system In placenta, it is present within invasive trophoblasts and in the surrounding extracellular space. Also present in amnion cells, but is not readily apparent in the extracellular matrix of this cell type. Present in normal pituitary gland and neoplastic pituitary gland (especially POMC-, GH- and PRL-producing adenomas) (at protein level). Widely expressed.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
CPZ
Also known as
CPZ; Carboxypeptidase Z; CPZ
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from CPZ . at AA range: 330-410
Observed band
73kD
Cellular localization
Secreted, extracellular space, extracellular matrix .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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