NT5C1A rabbit pAb from ELK Biotechnology Read more ›
NT5C1A rabbit pAb is a research-use antibody from ELK Biotechnology for studying NT5C1A in WB, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
Cytosolic nucleotidases, such as NT5C1A, dephosphorylate nucleoside monophosphates (Hunsucker et al., 2001 [PubMed 11133996]).[supplied by OMIM, Mar 2008],
| Specificity | NT5C1A Polyclonal Antibody detects endogenous levels of NT5C1A protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of A549 cells, using NT5C1A Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from MCF-7 and HepG2 cells, using NT5C1A Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES7766 |
| Target | NT5C1A |
| Research areas | >>Purine metabolism, >>Pyrimidine metabolism, >>Nicotinate and nicotinamide metabolism, >>Metabolic pathways, >>Nucleotide metabolism |
| Reactivity | Human,Mouse |
| Applications | WB,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NT5C1A. AA range:151-200 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Highly expressed in skeletal muscle. Detected at intermediate levels in heart, brain, kidney and pancreas. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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