ABM99564
TCF-3 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
TCF-3 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
TCF-3 rabbit pAb is an ELK Biotechnology antibody for research workflows involving TCF-3.
Background
This gene encodes a member of the T cell factor/lymphoid enhancer factor family of transcription factors. These transcription factors are activated by beta catenin, mediate the Wnt signaling pathway and are antagonized by the transforming growth factor beta signaling pathway. The encoded protein contains a high mobility group-box DNA binding domain and participates in the regulation of cell cycle genes and cellular senescence. [provided by RefSeq, Nov 2010],
Additional antibody information
| Specificity | TCF-3 Polyclonal Antibody detects endogenous levels of TCF-3 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of TCF7L1 Antibody. The lane on the right is blocked with the TCF7L1 peptide. | Western blot analysis of the lysates from HUVECcells using TCF7L1 antibody. |
Key Facts
Product type
Primary Antibody›
Target: TCF-3
| Target | TCF-3 |
| Also known as | TCF7L1; TCF3; Transcription factor 7-like 1; HMG box transcription factor 3; TCF-3 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse |
| Observed band | 63kD |
| Cellular localization | Nucleus. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Wnt signaling pathway;>>Hippo signaling pathway;>>Adherens junction;>>Melanogenesis;>>Cushing syndrome;>>Alcoholic liver disease;>>Salmonella infection;>>Human papillomavirus infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Pathways in cancer;>>Colorectal cancer;>>Endometrial cancer;>>Prostate cancer;>>Thyroid cancer;>>Basal cell carcinoma;>>Acute myeloid leukemia;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer;>>Arrhythmogenic right ventricular cardiomyopathy |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TCF7L1. AA range:321-370 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
TCF-3
Also known as
TCF7L1; TCF3; Transcription factor 7-like 1; HMG box transcription factor 3; TCF-3
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human TCF7L1. AA range:321-370
Observed band
63kD
Cellular localization
Nucleus.
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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