PSGR rabbit pAb from ELK Biotechnology Read more ›
PSGR rabbit pAb is a research-use antibody from ELK Biotechnology for studying PSGR in WB, IHC, ELISA workflows with listed reactivity for Human, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008],
| Specificity | PSGR Polyclonal Antibody detects endogenous levels of PSGR protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of A549 cells, using OR51E2 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from Jurkat cells, using OR51E2 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HepG2 cells using OR51E2 antibody. | Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES7633 |
| Target | PSGR |
| Research areas | >>Olfactory transduction |
| Reactivity | Human,Rat |
| Applications | WB,IHC,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human OR51E2. AA range:221-270 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Highly expressed in the prostate (PubMed:11707321). Also expressed in spleen, liver, olfactory epithelium, retinal pigment epithelium and medulla oblongata (PubMed:29249973, PubMed:11707321, PubMed:16491480). In the retinal pigment epithelium expression is restricted to the pigment cells and choroid (at protein level) (PubMed:29249973). Expressed in epidermal melanocytes (at protein level) (PubMed:27226631). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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