COL18A1 rabbit pAb
COL18A1 rabbit pAb from ELK Biotechnology Read more ›
Product Description
COL18A1 rabbit pAb is an ELK Biotechnology antibody for research workflows involving COL18A1.
Background
This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014],
Additional antibody information
| Specificity | COL18A1 Polyclonal Antibody detects endogenous levels of COL18A1 protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of A549 cells, using Collagen XVIII alpha1 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using Collagen XVIII alpha1 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HeLa cells using AIM2 antibody. |
Key Facts
| Target | COL18A1 |
| Also known as | COL18A1; Collagen alpha-1(XVIII) chain |
| Concentration | 1 mg/ml |
| Working concentration | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse |
| Cellular localization | Secreted, extracellular space, extracellular matrix . Secreted, extracellular space, extracellular matrix, basement membrane .; Non-collagenous domain 1: Secreted, extracellular space, extracellular matrix, basement membrane . Secreted .; Endostatin: Secreted . Secreted, extracellular space, extracellular matrix, basement membrane . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Protein digestion and absorption |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
IHCIFELISA |
| Dilution range | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Collagen XVIII alpha1. AA range:801-850 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.