ABM99496
VRK2 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
VRK2 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
VRK2 rabbit pAb is an ELK Biotechnology antibody for research workflows involving VRK2.
Background
vaccinia related kinase 2(VRK2) Homo sapiens This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. The encoded protein acts as an effector of signaling pathways that regulate apoptosis and tumor cell growth. Variants in this gene have been associated with schizophrenia. Alternative splicing results in multiple transcript variants that differ in their subcellular localization and biological activity. [provided by RefSeq, Jan 2014],
Additional antibody information
| Specificity | VRK2 Polyclonal Antibody detects endogenous levels of VRK2 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from Jurkat cells, using VRK2 Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
Product type
Primary Antibody›
Target: VRK2
| Target | VRK2 |
| Also known as | VRK2; Serine/threonine-protein kinase VRK2; Vaccinia-related kinase 2 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse |
| Observed band | 55kD |
| Cellular localization | Isoform 1: Cytoplasm . Endoplasmic reticulum membrane ; Single-pass type IV membrane protein . Mitochondrion membrane ; Single-pass type IV membrane protein . Nucleus envelope .; Isoform 2: Cytoplasm . Nucleus . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human VRK2. AA range:171-220 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
VRK2
Also known as
VRK2; Serine/threonine-protein kinase VRK2; Vaccinia-related kinase 2
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human VRK2. AA range:171-220
Observed band
55kD
Cellular localization
Isoform 1: Cytoplasm . Endoplasmic reticulum membrane ; Single-pass type IV membrane protein . Mitochondrion membrane ; Single-pass type IV membrane protein . Nucleus envelope .; Isoform 2: Cytoplasm . Nucleus .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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