Manufactured by:ELK Biotechnology
RUO

Tuberin (phospho Ser939) rabbit pAb

Tuberin (phospho Ser939) rabbit pAb from ELK Biotechnology

SKU:
ABM109341
Product type
Primary Antibody
TargetTuberin
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemLiver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Tuberin (phospho Ser939) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Tuberin in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Phospho-Tuberin (S939) Polyclonal Antibody detects endogenous levels of Tuberin protein only when phosphorylated at S939.
Validation evidence captions Western Blot analysis of 293 cells using Phospho-Tuberin (S939) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Tuberin/TSC2 (Phospho-Ser939) Antibody | Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Tuberin/TSC2 (Phospho-Ser939) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from 293 cells treated with Anisomycin 25ug/ml 30', using Tuberin/TSC2 (Phospho-Ser939) Antibody. The lane on the right is blocked with the phospho peptide.
Key facts & specifications
Research areas
>>Phospholipase D signaling pathway, >>p53 signaling pathway, >>Autophagy - animal, >>mTOR signaling pathway, >>PI3K-Akt signaling pathway, >>AMPK signaling pathway, >>Longevity regulating pathway, >>Cellular senescence, >>Thermogenesis, >>Insulin signaling pathway, >>Thyroid hormone signaling pathway, >>Human cytomegalovirus infection, >>Human papillomavirus infection, >>Herpes simplex virus 1 infection, >>Choline metabolism in cancer
Also known as
TSC2; TSC4; Tuberin; Tuberous sclerosis 2 protein
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human Tuberin/TSC2 around the phosphorylation site of Ser939. AA range:905-954
Observed band
200kD
Cellular localization
Cytoplasm. Membrane; Peripheral membrane protein. At steady state found in association with membranes.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 905-954
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES7448
UniProt entry
P49815
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00