PLC γ1 (phospho Tyr1253) rabbit pAb from ELK Biotechnology Read more ›
PLC γ1 (phospho Tyr1253) rabbit pAb is a research-use antibody from ELK Biotechnology for studying PLCG1 in WB, IHC workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
| Specificity | Phospho-PLC γ1 (Y1253) Polyclonal Antibody detects endogenous levels of PLC γ1 protein only when phosphorylated at Y1253. |
|---|---|
| Validation evidence captions | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PLCG1 (Phospho-Tyr1253) Antibody | Western blot analysis of lysates from LOVO cells treated with and 293 cells treated with heat shock, using PLCG1 (Phospho-Tyr1253) Antibody. The lane on the right is blocked with the phospho peptide. | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES6605 |
| Target | PLCG1 |
| Research areas | >>Inositol phosphate metabolism, >>Metabolic pathways, >>EGFR tyrosine kinase inhibitor resistance, >>ErbB signaling pathway, >>Ras signaling pathway, >>Rap1 signaling pathway, >>Calcium signaling pathway, >>Chemokine signaling pathway, >>NF-kappa B signaling pathway, >>HIF-1 signaling pathway, >>Phosphatidylinositol signaling system, >>Phospholipase D signaling pathway, >>Axon guidance, >>VEGF signaling pathway, >>Neutrophil extracellular trap formation, >>Natural killer cell mediated cytotoxicity, >>Th1 and Th2 cell differentiation, >>Th17 cell differentiation, >>T cell receptor signaling pathway, >>Fc epsilon RI signaling pathway, >>Fc gamma R-mediated phagocytosis, >>Leukocyte transendothelial migration, >>Neurotrophin signaling pathway, >>Inflammatory mediator regulation of TRP channels, >>Thyroid hormone signaling pathway, >>AGE-RAGE signaling pathway in diabetic complications, >>Growth hormone synthesis, secretion and action, >>Parkinson disease, >>Pathways of neurodegeneration - multiple diseases, >>Vibrio |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PLCG1 around the phosphorylation site of Tyr1253. AA range:1221-1270 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Epithelium,Testis,Vein, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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