Manufactured by:ELK Biotechnology
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NGEP rabbit pAb

NGEP rabbit pAb from ELK Biotechnology

SKU:
ABM108992
Manufacturer SKU
ES6477
Product type
Primary Antibodies
TargetNGEP
ApplicationsWB, IHC, IF/ICC, ELISA
Species / reactivityHuman, Rat, Mouse
Expression systemSpecifically expressed in epithelial cells of the prostate (at protein level).
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

NGEP rabbit pAb is a research-use antibody from ELK Biotechnology for studying NGEP in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This prostate-specific gene encodes a cytoplasmic protein, as well as a polytopic membrane protein which may serve as a target in prostate cancer diagnosis and immunotherapy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2011],

Additional antibody information

Specificity NGEP Polyclonal Antibody detects endogenous levels of NGEP protein.
Validation evidence captions Immunofluorescence analysis of Hela cell. 1,NGEP Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min. | Western Blot analysis of 3T3 cells using NGEP Polyclonal Antibody diluted at 1:1000 | Immunofluorescence analysis of HepG2 cells, using TM16G Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue, using TM16G Antibody. The picture on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
Antibody Research
Also known as
ANO7; NGEP; PCANAP5; TMEM16G; Anoctamin-7; Dresden transmembrane protein of the prostate; D-TMPP; IPCA-5; New gene expressed in prostate; Prostate cancer-associated protein 5; Transmembrane protein 16G
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human TM16G. AA range:881-930
Observed band
105kD
Cellular localization
[Isoform 1]: Cell membrane ; Multi-pass membrane protein . Cell junction . Endoplasmic reticulum . Concentrates at sites of cell-cell contact (PubMed:17308099). Shows an intracellular localization according to PubMed:22075693 and PubMed:20056604. .; [Isoform 2]: Cytoplasm, cytosol .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 881-930
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Manufacturer SKU
ES6477
UniProt entry
Q6IWH7
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00£176.00