Integrin β2 (phospho Thr758) rabbit pAb from ELK Biotechnology Read more ›
Integrin β2 (phospho Thr758) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Integrin β2 in WB, IHC workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014],
| Specificity | Phospho-Integrin β2 (T758) Polyclonal Antibody detects endogenous levels of Integrin β2 protein only when phosphorylated at T758. |
|---|---|
| Validation evidence captions | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CD18/ITGB2 (Phospho-Thr758) Antibody | Immunofluorescence analysis of HepG2 cells, using CD18/ITGB2 (Phospho-Thr758) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using CD18/ITGB2 (Phospho-Thr758) Antibody. The lane on the right is blocked with the phospho peptide. | Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES5951 |
| Target | Integrin β2 |
| Research areas | >>Rap1 signaling pathway, >>Phagosome, >>Hippo signaling pathway, >>Cell adhesion molecules, >>Complement and coagulation cascades, >>Neutrophil extracellular trap formation, >>Natural killer cell mediated cytotoxicity, >>Leukocyte transendothelial migration, >>Regulation of actin cytoskeleton, >>Pertussis, >>Legionellosis, >>Leishmaniasis, >>Malaria, >>Amoebiasis, >>Staphylococcus aureus infection, >>Tuberculosis, >>Human T-cell leukemia virus 1 infection, >>Rheumatoid arthritis, >>Viral myocarditis |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CD18/ITGB2 around the phosphorylation site of Thr758. AA range:720-769 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Leukocytes (PubMed:23775590). Expressed in neutrophils (at protein level) (PubMed:21193407, PubMed:28807980). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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