Integrin β1 (phospho Tyr795) rabbit pAb from ELK Biotechnology Read more ›
Integrin β1 (phospho Tyr795) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Integrin β1 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
| Specificity | Phospho-Integrin β1 (Y795) Polyclonal Antibody detects endogenous levels of Integrin β1 protein only when phosphorylated at Y795. |
|---|---|
| Validation evidence captions | Western blot analysis of ITGB1 (Phospho-Tyr795) Antibody. The lane on the right is blocked with the ITGB1 (Phospho-Tyr795) peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5946 |
| Target | Integrin β1 |
| Research areas | >>Rap1 signaling pathway, >>Phagosome, >>PI3K-Akt signaling pathway, >>Axon guidance, >>Focal adhesion, >>ECM-receptor interaction, >>Cell adhesion molecules, >>Tight junction, >>Platelet activation, >>Leukocyte transendothelial migration, >>Regulation of actin cytoskeleton, >>Bacterial invasion of epithelial cells, >>Pathogenic Escherichia coli infection, >>Shigellosis, >>Pertussis, >>Yersinia infection, >>Leishmaniasis, >>Toxoplasmosis, >>Human papillomavirus infection, >>Pathways in cancer, >>Proteoglycans in cancer, >>Small cell lung cancer, >>Hypertrophic cardiomyopathy, >>Arrhythmogenic right ventricular cardiomyopathy, >>Dilated cardiomyopathy |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ITGB1 around the phosphorylation site of Tyr795. AA range:749-798 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Isoform 1: Widely expressed, other isoforms are generally coexpressed with a more restricted distribution. ; Isoform 2: Expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. ; Isoform 3: Together with isoform 4, is expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Expressed in non-proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. ; Isoform 4: Together with isoform 3, is expressed in muscle, kidney, liver, placenta, ce |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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